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Antimicrobial Resistance · 8 Gene Markers

Resistance Data That Arrives With the ID.

Carbapenemases, MRSA, vancomycin, ESBL, sulfonamide, and trimethoprim resistance — automatically reflexed from any positive bacterial primary panel.

At a glance
0
Resistance Genes Detected
0
Carbapenemase Genes
0
Extra Specimens Required
0
Hour TAT for results
The clinical problem
Resistance data should arrive with the ID — not three days later.

Phenotypic susceptibility takes 48–72 hours after a positive culture. By the time it lands, empirical therapy is locked in and stewardship is reacting, not guiding.

Eight actionable resistance genes — mecA/C, VanA, VanB, KPC, NDM, CTX-M, Sul1/2, DfrA — reflexed automatically and reported alongside the organism ID in the same 24–48 hour window.

By the Numbers

The clinical weight behind this panel.

Industry statistics from public-health bodies and peer-reviewed literature — context for why this testing matters.

2.8M+
antibiotic-resistant infections in the U.S. each year — 35K deaths
Source · CDC AR Threats 2019
$4.6B
annual U.S. healthcare cost attributable to AR infections
Source · CDC
10M
projected global deaths/year from AMR by 2050 without action
Source · WHO / O'Neill Review
>30 genes
covered including blaKPC, blaNDM, mecA, vanA — actionable at the molecule

Genotype anticipates phenotype faster than culture-based AST.

Figures reflect publicly reported epidemiology and clinical literature for context only. They are not performance claims for the Chaseville Labs assay. See individual citations from the U.S. Centers for Disease Control and Prevention, World Health Organization, National Institutes of Health, and peer-reviewed journals.

What This Panel Diagnoses

Conditions, syndromes & infections covered.

The clinical scenarios where this panel is the right call — built around the differential your providers are actually working through.

Multi-Drug Resistant Gram-Negatives

2
Carbapenem-Resistant Enterobacterales (CRE)

KPC, VIM, NDM, IMP, OXA-48-like — the WHO's highest-priority resistance threats. Often fatal in bloodstream infection.

Clinical Impact ·Detection mandates contact precautions, public-health notification, and immediate stewardship escalation. Drives ceftazidime-avibactam, meropenem-vaborbactam, or cefiderocol selection.
ESBL Producers (CTX-M)

Extended-spectrum beta-lactamases — common in E. coli and Klebsiella UTI and bloodstream infections.

Clinical Impact ·Avoid third-generation cephalosporins (ceftriaxone, cefotaxime) — they will fail. Carbapenems or beta-lactam / beta-lactamase inhibitor combinations preferred.

Gram-Positive Resistance

2
MRSA — Methicillin-Resistant S. aureus (mecA/C)

Direct gene-level confirmation of methicillin resistance — no waiting for phenotypic susceptibility.

Clinical Impact ·Switch from beta-lactams to vancomycin, daptomycin, linezolid, or ceftaroline depending on infection site. Same-day decision instead of same-week.
VRE — Vancomycin-Resistant Enterococcus (VanA, VanB)

Enterococcus faecium and E. faecalis with vancomycin resistance — a feared healthcare-associated pathogen.

Clinical Impact ·Drives linezolid or daptomycin selection and triggers contact precautions to prevent ward spread.

Other Actionable Resistance

3
Fluoroquinolone Resistance (QNR)

Predicts failure of ciprofloxacin, levofloxacin, and moxifloxacin — common in recurrent UTI and travel-associated infections.

Clinical Impact ·Avoid empirical fluoroquinolones — beta-lactams, fosfomycin, or nitrofurantoin for cystitis preferred.
TMP-SMX & Sulfonamide Resistance (Sul1, Sul2, DfrA)

Predicts trimethoprim-sulfamethoxazole (Bactrim) failure — relevant in UTI, SSTI, and prophylaxis.

Clinical Impact ·Avoid empirical Bactrim — drives alternative agent selection.
Empirical Therapy Failure

The patient not getting better on standard antibiotics — culture eventually shows resistance, but the patient has already deteriorated.

Clinical Impact ·ABR reflex turns a 72-hour stewardship conversation into a same-day decision.
Complete Target List

8 targets, resolved from a single specimen.

Each organism below is reported individually as Detected, Not Detected, or Inconclusive — grouped here by pathogen class for clinical scanability.

Carbapenem Resistance

2 targets
KPCNDM

MRSA Marker

1 target
mecA/C

Vancomycin Resistance

2 targets
VanAVanB

Sulfonamide Resistance

1 target
Sul1/2

ESBL

1 target
CTX-M

Trimethoprim Resistance

1 target
DfrA
Why Molecular

Culture was built for a different century.

Multiplex real-time PCR resolves what culture and rapid antigen miss — fastidious organisms, polymicrobial infections, viruses, and resistance markers — from a single specimen.

Conventional
The Old Way

Culture & rapid antigen

  • 3–5 days
    Patient empirically treated before any answer arrives
  • Misses fastidious & viral organisms
    No growth ≠ no infection
  • Single-organism bias
    Polymicrobial infections under-reported
  • No resistance data
    Susceptibilities arrive a day later, if at all
  • Specimen-quality dependent
    Pre-treated patients culture negative
Multiplex PCR
The CRL Way

ABR Reflex on Bio-Rad CFX384

  • 24–48 hours
    Most reports back the next clinical day
  • 8 targets, one run
    Bacterial, viral, fungal, and parasitic in a single multiplex
  • Detects what culture can't
    Fastidious organisms, viruses, and polymicrobial infections, all reported individually
  • Validated LDT
    CLIA-certified, internally controlled, per-target Detected / Not Detected reporting
Primary Specimen
Same as triggering primary panel
Collection Container
Same as triggering primary panel
Volume
From primary specimen aliquot
Storage
Same as triggering primary panel
Transport
Pre-paid FedEx Priority Overnight
Stability
Validated per storage condition
Chain of Custody
Barcoded, temperature-logged in transit
Result Format

Per-target results, with clinical context.

Every analyte is reported individually as Detected, Not Detected, or Inconclusive. What a Detected result means clinically depends on which category the target falls into:

Carbapenem Resistance
KPCNDM
If Detected

KPC, VIM, NDM, IMP, and OXA-48-like detection indicates carbapenem-resistant Enterobacterales — contact precautions, public-health notification, and consideration of newer beta-lactam/beta-lactamase inhibitors or cefiderocol.

MRSA Marker
mecA/C
If Detected

mecA/C detection confirms methicillin resistance — switch from beta-lactams to vancomycin, daptomycin, linezolid, or ceftaroline depending on site.

Vancomycin Resistance
VanAVanB
If Detected

VanA/VanB detection indicates vancomycin-resistant Enterococcus — treat with linezolid or daptomycin and apply contact precautions.

Sulfonamide Resistance
Sul1/2
If Detected

Sul1/Sul2 detection predicts TMP-SMX failure for the carried organism.

ESBL
CTX-M
If Detected

CTX-M detection identifies extended-spectrum beta-lactamase producers — avoid third-generation cephalosporins; carbapenems or BL/BLI combinations are preferred.

Trimethoprim Resistance
DfrA
If Detected

DfrA detection predicts trimethoprim failure and supports alternative agents for UTI or skin/soft-tissue infection.

Qualitative multiplex real-time PCR (Bio-Rad CFX384)
Turnaround: 24–48 hours (added to primary panel result)